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解磷微生物在提高土壤肥力及促进作物对磷的吸收方面具有重要作用,明确其利用难溶性无机磷的本质在实际应用中具有重要的意义。分别在以Ca3(PO4)2和K2HPO4为唯一磷源的培养基中,检测了PSB28的解磷能力,并比较了生长动态和代谢产物的方面差异以分析其代谢难溶性无机磷的机理。结果表明,与含K2HPO4的培养基相比,在以Ca3(PO4)2为唯一磷源的培养基中菌体生长量较大但生长速率较慢,pH略高,呈逐渐降低趋势。HPLC测定结果发现,有机酸种类和含量变化明显,且酸性磷酸酶活性明显增强。因此菌株PSB28解磷的主要原因是有机酸的螯合作用,酸性磷酸酶起到间接作用。
Phosphate-solubilizing microorganisms play an important role in improving soil fertility and promoting the uptake of phosphorus by crops. It is of great significance to clarify the nature of the use of insoluble inorganic phosphorus in practical applications. The ability of phosphorus-solubilizing of PSB28 was detected in the medium with Ca3 (PO4) 2 and K2HPO4 as the sole phosphorus source, and the differences of growth dynamics and metabolites were compared to analyze the mechanism of the metabolism of insoluble inorganic phosphorus. The results showed that compared with K2HPO4-containing medium, the growth rate of the medium was higher than that of the medium containing K2HPO4, but the growth rate was slower and the pH was slightly higher, showing a gradually decreasing trend. The results of HPLC showed that the types and contents of organic acids changed significantly, and the activity of acid phosphatase increased obviously. Therefore, the main reason for the strain PSB28 to dissolve phosphorus is the chelation of organic acids, and the acid phosphatase plays an indirect role.