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目的 探讨外周血淋巴细胞 (PBL)中金属硫蛋白 (MT)基因表达作为镉接触生物标志物的可行性。方法 选择职业接触镉的人群 ,进行接触评定和效应评定 ,分离淋巴细胞进行体外培养 ,测定MT基因表达水平。结果 镉接触工人PBL的基础、诱导的MT表达水平分别为 1.5 7±0 .5 7、3.6 2± 1.0 2 ,明显高于对照组 (1.2 4± 0 .45、2 .6 8± 0 .78) ,差异有显著性 (P <0 .0 5 )。随尿镉、血镉和镉摄入量水平上升 ,镉接触工人PBL的MT基础表达和诱导表达都增高。MT基础和诱导表达与血镉水平显著相关 (偏相关系数分别为 0 .493和 0 .5 91) ;同时与尿镉也显著相关 (偏相关系数分别为 0 .314和 0 .334)。血镉水平与MT基础表达和诱导表达存在剂量 -效应关系。结论 PBL中MT基因表达作为镉接触的生物标志物是可行的
Objective To investigate the feasibility of using metallothionein (MT) gene in peripheral blood lymphocytes (PBLs) as biomarkers of cadmium exposure. Methods The population of occupational exposure to cadmium was selected for contact assessment and evaluation of effects. Lymphocytes were isolated and cultured in vitro. MT gene expression levels were determined. Results The level of MT expression induced by cadmium exposed workers was 1.57 ± 0.57 and 3.6 ± 1.02 respectively, which was significantly higher than that of the control group (1.2 4 ± 0.45 and 2.68 ± 0.78 ), The difference was significant (P <0. 05). With urinary cadmium, blood cadmium and cadmium intake levels rise, cadmium exposure workers PBL MT basic and induced expression were increased. The basal and induced expression of MT was significantly associated with serum cadmium levels (partial correlation coefficients of 0.493 and 0.51, respectively), and significant correlation with urinary cadmium (partial correlation coefficient of 0.334 and 0.334, respectively). There is dose-effect relationship between blood cadmium level and basic and induced MT expression. Conclusion MT gene expression in PBL is feasible as a biomarker for cadmium exposure