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用荧光探针NBD-C6-HPC标记体外培养48h的小鼠肝细胞,细胞膜磷脂用570型粘附式细胞仪激光漂白局部细胞膜,然后测定荧光的重新分布。结果显示,荧光恢复牢为76/,膜流动性为(1.49±0.057)×10 ̄(-9)cm ̄2/s。
Mouse hepatocytes cultured in vitro for 48 h were labeled with the fluorescent probe NBD-C6-HPC, and the cell membrane phospholipids were laser-bleached into the local cell membrane using a 570-type adherent cytometer and then the fluorescence redistribution was measured. The results showed that the fluorescence recovery was 76% and the membrane fluidity was (1.49 ± 0.057) × 10 -9 cm 2 / s.