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通过施加外源Me JA,SA及2种内生菌处理,研究白及组培苗生理及总酚含量的影响。采用组培的方法将种子培养为无菌苗,再对其施加不同处理,观察及测定生理和总酚含量的变化。结果发现:SA各浓度处理下幼苗生长情况均较差,40μmol·L~(-1)Me JA,50 m L·L~(-1)Hypocrea koningii及10 m L·L~(-1)Trichoderma koningiopsis处理下幼苗生长状况较好;各浓度SA处理下,SOD,POD及CAT活性均较高,Me JA处理下SOD,POD在较高浓度时活性较高,CAT活性则在80μmol·L~(-1)时较高,H.koningii处理下SOD,POD活性均随处理浓度的升高而升高,CAT活性则在1 m L·L~(-1)时较高,T.koningiopsis处理下SOD,POD,CAT活性均随浓度升高先升高后降低,均在10 m L·L~(-1)时较高。4种处理下MDA、可溶性蛋白及脯氨酸含量均有不同程度的升高;60μmol·L~(-1)Me JA处理下幼苗多糖含量较高;40μmol·L~(-1)Me JA,60μmol·L~(-1)SA,1 m L·L~(-1)H.koningii及10 m L·L~(-1),T.koningiopsis处理下总酚含量较高。说明添加一定浓度的外源Me JA,SA及内生菌处理均能在一定程度上提高白及幼苗的抗逆性、提高总酚含量,一定浓度下Me JA及2种内生菌处理还具有促进幼苗生长的作用。
The effects of exogenous Me JA, SA and two kinds of endophytic bacteria on the physiological and total phenolic content of white and tissue culture seedling were studied. The method of tissue culture was used to seed the seedlings as aseptic seedlings. Different treatments were applied to the seedlings to observe and determine the changes of physiological and total phenolic contents. The results showed that the growth of seedlings under SA stress was poor with 40 μmol·L -1 Me JA, 50 m L·L -1 Hypocrea koningii and 10 m L·L -1 Trichoderma Under the treatment of konjac and koningiopsis, the seedling growth was better. Under the SA treatment, the activities of SOD, POD and CAT were higher. Under the treatment of Me JA, the activities of SOD and POD were higher at higher concentration and CAT activity was at 80μmol·L ~ -1). The activities of SOD and POD in H.koningii increased with the increase of treatment concentration, and the CAT activity was higher at 1 m L · L -1. Under the treatment of T.koningiopsis The activity of SOD, POD and CAT increased first and then decreased with the increase of the concentration, which was higher at 10 m L · L -1. The contents of MDA, soluble protein and proline in the four treatments increased to different extents. The content of polysaccharide in the seedlings treated with 60μmol·L -1 Me JA was higher than that in the other two treatments. Me JA at 40μmol·L -1, The total phenols content of 60 μmol·L -1 SA, 1 m L·L -1 H.koningii and 10 m L·L -1 T.koningiopsis treatments were higher. These results indicated that adding exogenous Me JA, SA and endophytic bacteria could increase the stress resistance of white and seedlings to a certain extent and increase the total phenolic content. Me JA and two endophytic bacteria treatments also had Promote seedling growth.