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目的观察输注含游离血红蛋白(FHb)血液后对中度失血大鼠重要脏器的损伤和功能的影响,为科学制定战时输血质量标准的进一步研究提供实验基础。方法用SD大鼠建立中度失血模型,输入含不同剂量FHb的血液。根据每千克体重输注FHb的量将实验分为7组(组1~组7 FHb的输注量分别为100,80,60,40,20,10,0mg/kg);检测实验大鼠心、肝和肾功能的生化指标;测定血、尿标本FHb含量;对实验后48 h大鼠的心脏,肝脏,肾脏,脾脏和肺脏进行病理分析。结果组1实验大鼠于输血后8~12 h死亡,其他剂量组大鼠实验后48 h内均无死亡。心肌酶(GOT,U/L)检测结果,组2至组7分别为:322.1±7.39,119.8±2.19,104.6±10.06,73.9±5.46和64.4±10.24,63.3±6.82(对照组);肝功能(GPT,U/L)检测结果,组2至组7分别为:86.5±6.04,70.8±8.22,43.8±5.08,38.9±4.89,30.6±3.04,36.2±3.37(对照组);肾功能(BUN和Cr)检测结果均在正常值范围波动。病理结果:组2、组3和组4心脏和肝脏均出现病理改变,组5、组6和组7未见病理变化;组2、组3、组4和组5肾脏均出现病理改变,组6和组7未见病理变化;脾脏与肺脏各组均未见明显病理改变。结论中度失血大鼠输入含10 mg/kg FHb的血液为输血安全剂量。
Objective To observe the effects of transfusion of free hemoglobin (FHb) on the injury and function of important organs in rats with moderate blood loss, and provide the experimental basis for further research on the development of wartime transfusion quality standards. Methods A moderate blood loss model was established in SD rats, and blood containing different doses of FHb was input. According to the amount of FHb infused per kg body weight, the experiment was divided into 7 groups (100, 80, 60, 40, 20, 10 and 0 mg / kg respectively for group 1 to group 7 FHb) , Liver and kidney function; determination of FHb content in blood and urine samples; pathological analysis of heart, liver, kidney, spleen and lungs of rats 48 h after the experiment. Results Group 1 rats died 8-12 h after blood transfusion, while rats in other dosage groups did not die within 48 h after the experiment. Myocardial enzyme (GOT, U / L) test results, group 2 to group 7 were: 322.1 ± 7.39,119.8 ± 2.19,104.6 ± 10.06,73.9 ± 5.46 and 64.4 ± 10.24,63.3 ± 6.82 (control group); liver function (GPT, U / L). The results of group 2 to group 7 were: 86.5 ± 6.04,70.8 ± 8.22,43.8 ± 5.08,38.9 ± 4.89,30.6 ± 3.04,36.2 ± 3.37 (control group) And Cr) test results in the normal range of fluctuations. Pathological results: Pathological changes were observed in group 2, group 3 and group 4 heart and liver, and group 5, group 6 and group 7 showed no pathological changes; group 2, group 3, group 4 and group 5 had pathological changes in kidney, No pathological changes were observed in group 6 and group 7. No obvious pathological changes were observed in spleen and lung. Conclusion The moderate blood loss rats fed with 10 mg / kg FHb blood transfusion safety dose.