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目的通过检测大鼠血清中肿瘤坏死因子-α(TNF-α)的浓度,观察沙利度胺治疗由2,4,6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎(UC)的疗效。方法 21只TNBS结肠炎动物模型,随机分为模型组、柳氮磺吡啶组及沙利度胺组,每组7只。模型组不给予干预、沙利度胺组给予沙利度胺200 mg/(kg·d)、柳氮磺吡啶组给予柳氮磺吡啶100 mg/(kg·d),测定大鼠血清中TNF-a的浓度,探讨沙利度胺对UC的疗效。结果沙利度胺组大鼠腹泻次数减少,部分有隐血阳性,一直未出现肉眼血便。沙利度胺组、柳氮磺吡啶组、模型组大鼠TNF-a的浓度分别为(19.51±8.06)、(40.88±14.24)、(115.32±9.60)pg/ml,沙利度胺组降低血清TNF-α浓度的幅度高于柳氮磺吡啶组,差异有统计学意义(P<0.01)。结论沙利度胺治疗UC有效,能显著减轻结肠炎症,降低TNF-α浓度强于柳氮磺吡啶。
Objective To detect the concentration of tumor necrosis factor-α (TNF-α) in rat serum and to observe the effect of thalidomide on UC-induced ulcerative colitis (UC )Efficacy. Methods Twenty-one TNBS-induced colitis animal models were randomly divided into model group, sulfasalazine group and thalidomide group, with 7 rats in each group. In the model group, thalidomide was given thalidomide 200 mg / (kg · d), sulfasalazine was given sulfasalazine 100 mg / (kg · d), and the level of TNF -a concentration, to explore the efficacy of thalidomide on UC. Results The number of diarrhea in the thalidomide group was decreased, and some were occult blood test. No bloody stool appeared. The concentrations of TNF-α in thalidomide group, sulfasalazine group and model group were (19.51 ± 8.06), (40.88 ± 14.24), (115.32 ± 9.60) pg / ml, The magnitude of serum TNF-α was higher than that of sulfasalazine (P <0.01). Conclusions Thalidomide is effective in treating UC and can significantly reduce colonic inflammation and reduce the concentration of TNF-α in patients with sulfasalazine.