论文部分内容阅读
目的建立测定中药材中19种氨基甲酸酯农药残留量的超高效液相-串联四级杆质谱方法。方法多反应监测模式测定,以Waters ACQUITY UPLC(BEHC18进行分离,乙腈-0.1%甲酸溶液(1∶1),流速:0.2 mL.min-1。离子化模式:ESI+。结果 19种农药测定的线性范围在1.0～100.0μg.L-1之间,r分别在0.994 7～0.999 7之间,检出质量浓度分别在0.02～0.46μg.L-1之间,3个水平的回收率分别在73.2%～119.7%之间。结论该方法快速、准确、灵敏,可用于中药材中氨基甲酸酯类农药残留的测定。
Objective To establish a method for the determination of 19 carbamate pesticide residues in Chinese herbal medicines by ultra performance liquid chromatography-tandem quadrupole mass spectrometry. Method Multi-reaction monitoring assay was performed on a Waters ACQUITY UPLC (BEHC18, acetonitrile-0.1% formic acid solution (1: 1) with a flow rate of 0.2 mL min -1. Ionization mode: ESI + With the range of 1.0 ~ 100.0μg.L-1, r respectively ranging from 0.994 7 to 0.999 7, the detected concentrations were 0.02 ~ 0.46μg.L-1, the recoveries of the three levels were 73.2 % ~ 119.7% .Conclusion The method is rapid, accurate and sensitive and can be used for the determination of carbamate pesticide residues in Chinese herbal medicines.