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小鼠骨髓微核试验法是检测环境化学物质对细胞遗传损伤的一个非常有用指标。具有简易快速的特点。制片技术是该试验关键所在。目前国内外常用方法是用小牛血清冲洗骨髓腔或用止血钳夹碎股骨于小牛血清中,而后离心涂片。此法有两个缺点往往影响试验的成败。一是小牛血清的存放时间影响涂片的染色效果。存放时间越久,染色效果越差。可能在存放过程中,小牛血清中的糖脂分解变性,色泽和/或pH值发生变化所致。
Mouse bone marrow micronucleus test is a very useful indicator of environmental genetic damage to cells. Has a simple and quick features. Production technology is the key to this test. At present, the commonly used method at home and abroad is to wash the bone marrow cavity with calf serum or to clamp the femur in calf serum with a hemostatic forceps, and then centrifuge the smear. This method has two shortcomings often affect the success of the test. First, the storage time of bovine serum affect the smear results. The longer the storage time, the worse the staining effect. May be stored in the bovine serum glycolipid degeneration, color and / or pH changes due to.