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利用SDS-PAGE和HPLC-荧光检测法对大豆含硒蛋白或多肽进行分离和硒含量测定,在“>2SD”为含硒条带的约定下,从所采集恩施,北京,启东样品中分别检出了19,12和10条含硒蛋白或亚基条带,并初步估算了它们的分子量和含硒量。根据这些蛋白条带的归属分析,发现3个地区样品大豆乳清蛋白结合硒的量最多,11S组分结合硒的量次之,7S组分结合硒的量最少。经过对不同硒含量样品进行比较后认为,大豆乳清蛋白中高分子量组分是过量摄取硒的主要结合部位,中分子量组分的29.9 ku和26.3～26.9ku条带专属性结合硒,11S的A_3组分和酸性亚基按比较固定的比例结合硒.硒在蛋白或亚基中的分布性质是不均一的,存在明显地区间差异.
SDS-PAGE and HPLC-fluorescence detection of soybean selenium-containing protein or polypeptide separation and selenium content determination, “> 2SD” as the selenium band agreement, collected from Enshi, Beijing, Qidong samples were seized 19, 12 and 10 bands containing selenoproteins or subunits were identified and their molecular weights and selenium contents were initially estimated. Based on the attribution analysis of these protein bands, we found that soybean whey protein bound selenium in the three regions was the highest, followed by 11S with selenium and 7S with the least amount of selenium. After comparing the samples with different selenium content, it is concluded that the high molecular weight component of soybean whey protein is the main binding site of excess selenium. The middle molecular weight component of 29.9 ku and 26.3-26.9ku band exclusively bind selenium and 11S A_3 The components and acidic subunits bind selenium in relatively fixed proportions. The distribution nature of selenium in proteins or subunits is heterogeneous and there is a clear interval difference.