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pH区带精制逆流色谱法是在普通制备型高速逆流色谱分离纯化生物碱类成分的应用基础上发展起来的一种特殊逆流色谱分离制备技术。pH区带精制逆流色谱法由于具备进样量高、分离效率高、分离效果好、杂质易收集、可实现pH监控、待分离组分被高度浓缩等优点,目前已被广泛应用于研究领域与实践中。生物碱类物质是重要的天然产物成分,具有很强的生物活性,对很多疾病有良好的治疗作用。本文介绍了pH区带精制逆流色谱法及其在分离生物碱类天然产物中的应用。
The pH-zone countercurrent chromatography is a special counter-current chromatographic separation and preparative technique developed on the basis of the application of common preparative high-speed countercurrent chromatography to separate and purify alkaloids. Because of the advantages of high injection volume, high separation efficiency, good separation effect, easy collection of impurities, pH monitoring and high concentration of the separated components, pH zone refined countercurrent chromatography has been widely used in the field of research Practice. Alkaloids are important natural product components, have strong biological activity, and have good therapeutic effect on many diseases. This article describes the pH zone with countercurrent chromatography and its application in the separation of alkaloids in natural products.