目的 :为了探讨直肠癌细胞增殖情况与其预后及淋巴结转移的关系。方法 :本文通过对 30例Ⅲ期直肠癌 (DUKSC期 )石蜡包埋组织块及 10例正常组织对照石蜡块 ,进行细胞悬液制备 ,并采用B -D公司生产的FACSCalibur型流式细胞仪 ,进行细胞获取和分析。结果 :①正常 10例直肠组织细胞G0 -G1期细胞峰值道数为 49 82± 1 80。②DI(DNA指数 )、SPF(S期细胞百分率 )、PI(细胞增殖指数 )与淋巴结转移情况无关 (P>0 0 5 )。③SPF与直肠癌 5年生存率关系密切 (P <0 0 1)。结论 :S期细胞百分率越高预后越差 ,而DI、PI与预后无关。 Objective: To investigate the relationship between the proliferation of rectal cancer cells and its prognosis and lymph node metastasis. METHODS: Thirty cases of stage III rectal cancer (DUKSC phase) paraffin-embedded tissue blocks and 10 normal tissue control paraffin blocks were prepared for cell suspension preparation using the FACSCalibur flow cytometer produced by B-D Corporation. Cell acquisition and analysis were performed. RESULTS: 1 The peak number of G0-G1 phase cells in normal 10 cases of rectal tissue was 49 82±180. 2DI (DNA index), SPF (percentage of S-phase cells), PI (cell proliferation index) were not related to lymph node metastasis (P>0.05). 3SPF was closely related to the 5-year survival rate of rectal cancer (P < 0 01). Conclusion: The higher the percentage of S-phase cells, the worse the prognosis, and DI and PI have nothing to do with the prognosis.