论文部分内容阅读
目的探索mi R-21联合癌胚抗原(CEA)对结肠癌的诊断意义,为结肠癌早期检测提供新的依据。方法选择2010年12月至2013年12月于收治的结肠癌患者92例,匹配正常人88例;采集一般信息包括年龄、性别和CEA表达情况等,提取血浆中RNA并用Q-PCR检测mi R-21的表达情况;分别用ROC曲线检测CEA、mi R-21、mi R-21联合CEA对结肠癌的诊断价值。结果正常人组CEA水平为(1.793±1.121)ng/m L,结肠癌患者CEA水平为(9.843±8.430)ng/m L,两组比较差异具有统计学意义(P<0.05)。Q-PCR结果显示结肠癌患者mi R-21表达水平是正常人的(5.510±3.971)倍,差异具有统计学意义(P<0.05)。ROC曲线分析结果显示,CEA组曲线下面积为(0.838±0.029)(95%CI 0.781-0.894),mi R-21组曲线下面积为(0.724±0.038)(95%CI 0.649-0.798),mi R-21联合CEA组曲线下面积(0.916±0.019)(95%CI0.878-0.953)。结论 mi R-21联合CEA优于CEA或mi R-21单独对于结肠癌的诊断价值,为临床早期检测结肠癌提供了新的依据。
Objective To explore the diagnostic value of mi R-21 combined carcinoembryonic antigen (CEA) in colon cancer and to provide a new basis for the early detection of colon cancer. Methods A total of 92 patients with colon cancer were enrolled from December 2010 to December 2013, 88 matched for normal subjects. General information including age, sex and CEA expression were collected and plasma RNA was extracted and detected by q-PCR The expression of CEA, mi R-21 and mi R-21 combined with CEA in colon cancer were detected by ROC curve respectively. Results The CEA level was (1.793 ± 1.121) ng / m L in normal controls and 9.843 ± 8.430 ng / m L in colon cancer patients. There was significant difference between the two groups (P <0.05). The results of Q-PCR showed that the expression of mi R-21 in colon cancer patients was (5.510 ± 3.971) times of that of normal people, the difference was statistically significant (P <0.05). ROC curve analysis showed that the area under the curve of the CEA group was (0.838 ± 0.029) (95% CI 0.781-0.894), and the area under the curve of the mi R-21 group was (0.724 ± 0.038) (95% CI 0.649-0.798) The area under the curve for R-21 in combination with CEA was (0.916 ± 0.019) (95% CI 0.878-0.953). Conclusion The diagnostic value of mi R-21 combined with CEA over CEA or mi R-21 alone for colon cancer provides a new basis for clinical detection of colon cancer.