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Objective: To elucidate the mechanism of vascular hyporeactivity following severe hemorrhagic shock (HS) by studying the changes of ATP-sensitive potassium channels(KATP) properties and membrane potential of mesenteric arteriolar smooth muscle cells.Methods: Single channel currents were studied on cell-attached and inside-out patches of enzymatically isolated mesenteric arteriolar smooth muscle cells (ASMCs). Membrane potentials of arteriolar strips and ASMCs were recorded by intracellular membrane potential recording method and confocal microscopy, respectively.Results: KATP channels in ASMCs were activated,which induced smooth muscle hyperpolarization following vsscular hyporeactivity in HS.Conclusions: Hyperpolarizing effect of KATP channel activation plays an important role in low vasoreactivity during severe hemorrhagic shock.