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基因标记和光子学显象技术相结合，实时观测中性粒细胞吞噬病原体细胞骨架运动规律． 利用致冷ＣＣＤ系统组建了高灵敏度的荧光激发与测试装置． 将带有绿色荧光蛋白（ＧＦＰ）基因的ＰＲＳＥＴＢ质粒转化大肠杆菌ＢＬ２１，利用ＢＬ２１高效表达ＧＦＰ后发出强烈绿色荧光的特点，用光子显象技术连续记录荧光的变化，从而在不受干扰的条件下实时动态观察了中性粒细胞粘附、内吞、消化大肠杆菌的全过程．
Gene markers and photon imaging techniques combined real-time observation of neutrophil phagocytosis of pathogens cytoskeleton movement. A high-sensitivity fluorescence excitation and test device was set up by the refrigeration CCD system. The PRSETB plasmid with green fluorescent protein (GFP) gene was transformed into E.coli BL21. The green fluorescent protein (GFP) was highly expressed by BL21, and the fluorescence was continuously recorded by photon imaging. Under the condition of no interference Real-time dynamic observation of the neutrophil adhesion, endocytosis, digestion of the entire process of E. coli.