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目的通过研究干扰素/维甲酸联合应用诱导细胞凋亡相关基因(GRIM-19)在小鼠植入前胚胎中的表达及其与胚胎质量之间的相关性,探讨GRIM-19在小鼠植入前胚胎发育中的作用。方法采用实时定量PCR,检测小鼠植入前胚胎(2-细胞期、4-细胞期、8-细胞期、桑葚胚和囊胚)中GRIM-19 mRNA水平(n=16);将小鼠8-细胞期胚胎按卵裂球大小、形态、透明带、胞质碎片分为优胚组和非优胚组,分别检测两组胚胎GRIM-19mRNA水平,并分析其相关性(n=13);制作假孕鼠(23只),并随机分为A组(12只)和B组(11只),采用移植器将优质和非优质8-细胞期胚胎移入假孕鼠的子宫内,观察两组雌鼠妊娠率及产仔率。结果 GRIM-19在小鼠植入前各期胚胎中均有表达,其mRNA水平从2-细胞期逐渐增高,至8-细胞期达高峰,随后呈下降趋势。优质8-细胞胚胎的GRIM-19 mRNA水平明显高于非优胚组(P<0.05)。两组8-细胞胚胎移植后,A组雌鼠妊娠率及产仔率显著高于B组雌鼠(P<0.05)。结论小鼠植入前胚胎中GRIM-19的表达量随发育时程的改变而变化,且与胚胎质量密切相关。
Objective To investigate the expression of GRIM-19 in mouse preimplantation embryos and its correlation with embryo quality by investigating the combined use of interferon / retinoic acid (RA) The role of preimplantation embryo development. Methods The level of GRIM-19 mRNA in preimplantation embryos (2-cell stage, 4-cell stage, 8-cell stage, morula and blastocysts) was detected by real- The 8-cell embryos were divided into two groups according to the size, morphology, zona pellucida and cytoplasmic fragments of blastocysts. The GRIM-19 mRNA levels in embryos were detected and analyzed (n = 13) (23 mice) were randomly divided into group A (n = 12) and group B (n = 11). Transplanted mice were used to transfer high quality and non-high quality 8- Pregnancy rate and litter size of two groups of females. Results GRIM-19 was expressed in all preimplantation embryos of mice. The mRNA level of GRIM-19 increased gradually from the 2-cell stage to the 8-cell stage and then decreased. The level of GRIM-19 mRNA in high quality 8-cell embryos was significantly higher than that in non-excellent embryos (P <0.05). After the two groups of 8-cell embryos were transplanted, the pregnancy rate and litter size in group A were significantly higher than those in group B (P <0.05). Conclusion The expression of GRIM-19 in preimplantation embryos of mice varies with the developmental time course, and is closely related to the embryo quality.