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目的研究细胞免疫功能和细胞因子白细胞介素６（ＩＬ－６）在创伤及修复过程中的作用与临床的关系。方法观察１４例重度和２８例轻度创伤患者Ｔ细胞体外培养增殖反应、Ｔ细胞亚群的变化与ＩＬ－６的表达。结果５７．１４％的重度创伤患者Ｔ细胞增殖反应减弱，ＩＬ－６的表达受到抑制，Ｔ细胞体外培养液中ＩＬ－６含量降低为８２．３６ｐｇ／ｍｌ，而轻度创伤和健康对照组分别为１８３．０７ｐｇ／ｍｌ和４７２．９０ｐｇ／ｍｌ，其差异有非常显著性意义。重度创伤患者ＣＤ８＋细胞数量相对增加，百分率增高为３３．４３％，使ＣＤ４＋／ＣＤ８＋比值下降（１５８），明显低于轻度创伤（１．７３）和健康对照组（１．７６），差异有显著性意义。细胞培养液中ＩＬ－６的含量与ＣＤ８＋细胞数量呈负相关（ｒ＝－０．４３８）。结论在重度创伤患者ＣＤ８＋细胞数相对增多时，可抑制Ｔ细胞的增殖能力，并伴有Ｔ细胞体外培养ＩＬ－６表达作用的减弱。
Objective To investigate the role of cellular immunity and cytokines interleukin-6 (IL-6) in trauma and repair and its clinical significance. Methods T cell proliferation, T lymphocyte subsets and IL-6 expression in 14 severe and 28 mild trauma patients were observed. Results 57.14% patients with severe trauma had weakened T cell proliferative response and suppressed the expression of IL-6. The level of IL-6 in T cells decreased to 82.36pg / ml, while mild trauma and healthy control group 183.07pg / ml and 472.90pg / ml, the difference has a very significant significance. The number of CD8 + cells in patients with severe trauma increased relatively, the percentage increased by 33.43%, the ratio of CD4 + / CD8 + decreased (158), which was significantly lower than mild trauma (1.73) and healthy control group (1.76) Significant significance. The content of IL-6 in cell culture medium was negatively correlated with the number of CD8 + cells (r = -0.438). Conclusions The relative increase of CD8 + cells in severe trauma patients can inhibit the proliferation of T cells and decrease the expression of IL-6 in T cells.