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研究丝裂霉素C(MitomycinC,MMC)的衍生物-新型吲哚醌类生物还原活性物629的细胞毒性和辐射增敏作用。采用四氮甲基唑蓝(Methylthiazolyltetrazolium,MMT)比色法研究MMC和629对HepG2细胞的细胞毒性以及629对肝原代细胞的细胞毒性,结果表明629对肝原代细胞无毒性作用,而其对HepG2肝癌细胞的毒性要高于其母体化合物MMC;此外,采用体外细胞克隆培养法观察MMC和629对HepG2细胞的辐射增敏作用,结果显示629的辐射增敏效果强于MMC。上述研究提示,629具有低毒的增敏效果,在临床上将具有广泛的应用远景。
To study the cytotoxicity and radiosensitization of mitomycin C (MMC), a novel indoquinone bioreductive compound 629, Methylthiazolyltetrazolium (MMT) colorimetric method was used to study the cytotoxicity of MMC and 629 to HepG2 cells and the cytotoxicity of 629 to primary hepatocytes. The results showed that 629 had no toxic effect on hepatoma cells, The cytotoxicity of HepG2 cells to HepG2 hepatoma cells was higher than that of the parent compound MMC. In addition, the radiation sensitization effect of MMC and 629 on HepG2 cells was observed by in vitro cell cloning and culture. The results showed that the radiosensitization of 629 was stronger than that of MMC. The above studies suggest that 629 has a low toxicity sensitizing effect, will have a wide range of clinical applications.