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以猥实(Kolkwitzia amabilis Graebn)枝条为外植体,探讨影响猥实分化的主要因素,通过正交实验结果表明:影响猥实分化的主要因素是培养基中的植物生长素和分裂素的浓度比值,诱导猥实启动的培养基为MS+6-BA 1.0 mg/L+NAA0.05 mg/L+糖30 mg/L+琼脂6.5 g/L,诱导不定芽的增殖培养基为MS+6-BA 1.2 mg/L+NAA 0.1 mg/L+糖30 g/L+琼脂6.5 g/L,蔗糖对猥实的分化影响效果不明显。
In order to explore the main factors that affect the differentiation of adulthood, the shoots of Kolkwitzia amabilis Graebn were used as explants. The orthogonal experimental results showed that the main factors affecting the differentiation of adulthood were the concentrations of auxin and mitogen The MS medium supplemented with 6-BA 1.0 mg / L + NAA 0.05 mg / L + sugar 30 mg / L + agar 6.5 g / L and the medium for induction of adventitious buds were MS + 6-BA 1.2 mg / L + NAA 0.1 mg / L + sugar 30 g / L + agar 6.5 g / L.