论文部分内容阅读
目的了解慢性乙型肝炎患者HBV前S1抗原(preS1-Ag)与HBV-M、HBV-DNA之间的相关性,探讨preS1-Ag检测的临床意义。方法用ELISA法对568份慢性乙型肝炎患者血清进行HBV标志物及preS1-Ag检测,同时采用荧光定量PCR法检测HBV-DNA。结果在HBsAg阴性血清中,preS1-Ag检测结果均为阴性;在HBsAg阳性血清中,HBeAg阳性时preS1-Ag的阳性率为84.6%,HBeAg阴性时preS1-Ag的阳性率为43.4%,两者之间差异具有显著性(χ2=95.3,P<0.01);HBV-DNA与preS1-Ag一致性比较Kappa=0.697,一致性较好;HBV-DNA与HBeAg一致性比较Kappa=0.345,一致性较差。结论preS1-Ag与HBeAg和HBV-DNA结果有较好的相关性,同时preS1-Ag与HBV-DNA结果的一致性优于HBeAg,preS1-Ag检测可作为判断HBV感染与复制的一项血清学标志物。
Objective To investigate the relationship between preS1-Ag and HBV-M, HBV-DNA in patients with chronic hepatitis B and to investigate the clinical significance of preS1-Ag detection. Methods HBV markers and preS1-Ag in serum of 568 patients with chronic hepatitis B were detected by ELISA, and HBV-DNA was detected by real-time quantitative PCR. Results The positive results of preS1-Ag in HBsAg-positive sera were 84.6% for HBeAg-positive sera and 43.4% for HBeAg-negative sera, respectively (Χ2 = 95.3, P <0.01). The concordance between HBV-DNA and preS1-Ag was better than that of preS1-Ag and Kappa = 0.697 difference. Conclusions PreS1-Ag has a good correlation with HBeAg and HBV-DNA results, and the preS1-Ag and HBV-DNA results are better than HBeAg. The preS1-Ag test can be used as a serological test to determine HBV infection and replication landmark.