以西洋参（ＰａｎａｘｑｕｉｎｑｕｅｆｏｌｉｕｍＬ．）的胚为材料，在附加ＧＡ３和ＩＢＡ的培养基上获得无菌苗。分别用含有Ｒｉ质粒的发根农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）１５８３４菌株及经过胡萝卜提取液处理后的该菌株感染无菌苗切段，在外加萘乙酸６ｍｇ／Ｌ的ＭＳ琼脂培养基培养后获得毛状根。胡萝卜处理组较未处理组转化率提高１倍，出根时间提前１０ｄ。转化根在不含激素的培养基上可快速生长，表现为典型的毛状根性状。ＰＣＲ分析证实内含５７７ｂｐｒｏｌＣ序列的ＴＬ－ＤＮＡ已整合人转化株的染色体组。 Ginseng (Panax quinquefolium L.) embryos as materials, GA3 and IBA added to the medium on the sterile seedlings. The Agrobacterium rhizogenes 15834 strain containing Ri plasmid and the strain treated with carrot extract were inoculated with sterile seedlings and cultured on MS agar medium supplemented with 6 mg / L NAA to obtain hairy roots . Carrot treatment group than the untreated group, the conversion rate increased by 1 time, the root out of time ahead of 10d. Transformed roots grew rapidly on hormone-free medium, showing typical hairy root traits. PCR analysis confirmed that the TL-DNA containing the 577 bpprol sequence has been integrated into the genome of human transformants.