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经门静脉给予受体LEW大鼠3×10~8个DA大鼠供体脾细胞,2d后腹腔注射80mg/kg的环磷酰胺,第4天由舌静脉输注1×10~8骨髓细胞。2周后实施心脏移植手术。观察、记录移植物的存活时间,通过过继性转移实验,MLR、CTL活性测定及嵌合体分析,探讨耐受机制。结果表明,异基因心脏移植物的存活时间进一步延长;该耐受状态可被过继性转移;MLR、CTL活性表明,受体大鼠的免疫应答被特异性抑制;嵌合体水平提高。结论:异基因骨髓细胞输注可加深由脾细胞和环磷酰胺诱导的大鼠心脏移植耐受,该耐受与嵌合体水平、抑制细胞的存在有关。输注骨髓细胞对于改善、维持耐受状态,延长大鼠心脏移植物存活时间是一有效的方法。
The recipient LEW rats received 3 × 10 ~ 8 DA rat donor spleen cells via the portal vein. Two days later, 80mg / kg cyclophosphamide was injected intraperitoneally. On the fourth day, 1 × 10 ~ 8 bone marrow cells were infused through the lingual vein. Two weeks after the implementation of heart transplant surgery. The survival time of the grafts was observed and recorded. The mechanism of tolerance was discussed by adoptive transfer assay, MLR, CTL activity assay and chimerism analysis. The results showed that the survival time of allogeneic heart grafts was further prolonged. The tolerated state could be adoptively transferred. The MLR and CTL activities showed that the immune response of the recipient rats was specifically inhibited and the level of chimera was increased. CONCLUSIONS: Allogeneic myeloid infusion enhances cardiac allograft tolerance induced by splenocytes and cyclophosphamide, which is associated with chimerism and suppression of the presence of cells. Infusion of bone marrow cells is an effective method to improve and maintain the tolerance status and prolong the survival time of cardiac allografts in rats.