[Objectives]This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb.at different harvest time,so as to select its best harvest time.[Methods]High performance liquid chromatography was used for the determination of agrimophol in A.pilosaLedeb.at different harvest time in Luzhou.[Results]The content of agrimophol could be detected by HPLC.Agrimophol had good linearity in 0.01-0.1mg/ml(r=0.999 9),the average recovery was 101.5%,and RSD was 1.10%.The content of agrimophol in A.pilosa Ledeb.in Luzhou was 0.010 8%in June,and 0.013 1%in July,much higher than any other months.[Conclusions]This experiment established a method for detecting the content of agrimophol by HPLC,which is simple,with high precision,high accuracy,good repeatability and recovery.The reasonable harvest time of A.pilosa Ledeb.in Luzhou is June and July. [Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb.at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agrimophol in A.pilosaLedeb.at different harvest time in Luzhou. [Results] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01-0.1 mg / ml (r = 0.999 9), the average recovery was 101.5%, and RSD was 1.10%. content of agrimophol in A.pilosa Ledeb.in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb.in Luzhou is June and July.