论文部分内容阅读
目的进一步探讨钙拮抗剂对进行性肾小球损伤保护作用的机制，本文观察了心痛定对５／６肾切除大鼠的治疗效应。方法：设置３个观察组：（１）对照组，进行５／６肾切除，术后喂正常饮食；（２）心痛定组，术后喂含０．２％心痛定的饲料；（３）依那普利组，术后喂含５０ｍｇ／Ｌ依那普利的水。分别观察血压，尿蛋白及肾功能的变化，并于术后第８周处死大鼠，测定其残余肾的组织形态学指标：系膜区面积，丝球体面积及体积和肾小球硬化指数。结果：心痛定组、依那普利组大鼠血压、尿蛋白、血浆尿素氮和肌酐均较对照组有所下降（Ｐ＜０．０１）；心痛定组系膜区面积和丝球体体积分别为（１．５３±０．４１）×１０３μｍ２、（１．２３±０．２７）×１０６μｍ３；较对照组（２．２４±０．５５）×１０３μｍ２和（１．５０±０．３３）×１０６μｍ３明显减少（Ｐ值均＜０．０１），依那普利组则无明显变化。结论：心痛定、依那普利均有降低肾衰模型大鼠血压、尿蛋白和氮质血症的作用，然而仅心痛定可减小大鼠肾小球系膜区面积和肾小球体积，从而减轻肾小球硬化的形成
Objective To further explore the protective effect of calcium antagonists on progressive glomerular injury, we observed the therapeutic effect of nifedipine on 5/6 nephrectomized rats. Methods: Three observation groups were set up: (1) control group, 5/6 nephrectomy and normal diet after operation; (2) nifedipine group, feed containing 0.2% nifedipine after operation; (3) In the enalapril group, water containing enalapril at 50 mg / L was given postoperatively. The changes of blood pressure, urinary protein and renal function were observed. At the 8th week after operation, the rats were sacrificed and the histomorphological parameters of the remnant kidney were measured: the mesangial area, the area and volume of the spheroids, and the glomerulosclerosis index. Results: Compared with the control group, the blood pressure, urinary protein, plasma urea nitrogen and creatinine of the rats in Xintongding group and Enalapril group were decreased (P <0.01); the area of mesangial membrane and the volume of silk spheres were (1.53 ± 0.41) × 103μm2 and (1.23 ± 0.27) × 106μm3, respectively. Compared with the control group (2.24 ± 0.55) × 103μm2 and (1.50 ± 0.33) × 106μm3 significantly reduced (P values were <0.01), no changes in the enalapril group. Conclusion: Both nifedipine and enalapril can reduce the blood pressure, urine protein and azotemia in rats with renal failure, however, only xintongding can reduce the area of rat mesangial area and glomerular volume , Thereby reducing the formation of glomerular sclerosis