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目的：观察放烧复合伤小鼠骨髓基质细胞贴壁层的粘附能力及对ＧＭ－ＣＦＵ（粒单系造血组细胞集落）形成的影响。方法：采用细胞粘附实验和细胞粘附阻断实验方法。检测５Ｇｙγ线全身照射、１５％体表面积Ⅲ度烧伤和放烧复合伤小鼠骨髓基质细胞粘附的功能。结果：单烧组伤后第３～７天显著高于正常组（Ｐ＜０．０５～０．０１）；单放组伤后第３～７天显著低于正常组（Ｐ＜０．０５～０．０１）；复合伤组伤后第３天显著低于正常组（Ｐ＜０．０１）；正常组骨髓基质细胞贴壁层分别加入ＶＣＡＭ－１、Ｆｎ、Ｌｎ、ＣｏｌⅣ等单抗或ＶＣＡＭ－１单抗分别联合Ｆｎ、Ｌｎ和ＣｏｌⅣ等单抗处理后，ＧＭ－ＣＦＵ数量均有不同程度的降低。结论：骨髓微环境基质细胞粘附功能的损伤可能是导致放烧复合伤造血功能障碍的重要原因之一
OBJECTIVE: To observe the adhesion of bone marrow stromal cells adherent layer and the effect on the formation of GM-CFU (granulocyte-only hematopoietic cell colonies) in combination burn-burn mice. Methods: Cell adhesion assay and cell adhesion assay were used. The function of 5Gyγ line whole body irradiation, 15% body surface area third degree burn and burn and burn combined bone marrow stromal cells adhesion function were detected. Results: The single burn group was significantly higher than the normal group on the 3rd to 7th day after injury (P <0.05 ~ 0.01). The single burn group was significantly lower on the 3rd to 7th day than the normal group (P <0.05) ~ 0.01). The composite wounds were significantly lower than the normal group on the 3rd day after injury (P <0.01). The normal group was added with VCAM-1, Fn, Ln, VCAM-1 mAb combined with Fn, Ln and Col IV and other monoclonal antibodies, respectively, the GM-CFU number decreased to varying degrees. Conclusion: The damage of bone marrow microenvironment stromal cell adhesion function may be one of the important reasons for the hematopoietic dysfunction caused by radiation and burn injury