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通过对OP(聚乙二醇辛基苯基醚)乳化剂的荧光光谱研究,发现其在304nm处产生较强的荧光。在pH 5.5的B-R缓冲溶液中,OP乳化剂的荧光强度与其浓度在一定范围内呈良好的线性关系,据此建立了一种新的高灵敏度的测定方法。在0.1—50.0μg/mL的浓度范围内,测定波长λex/λem=226nm/304nm的回归方程为:F=22.12C+9.825(C:μg/mL,r=0.9995),检出限为0.068μg/mL;测定波长λex/λem=276nm/304nm的回归方程为:F=90.08C-10.12(C:μg/mL,r=0.9999),检出限为0.053μg/mL。采用此两组测定波长对同组样品进行测定,样品回收率均在96.5%以上,对测定结果进行F检验和t检验分析,得出两组数据之间无显著性差异。方法灵敏度高,抗干扰能力较强,可用于测定较复杂环境样品中微量OP乳化剂含量。
Fluorescence spectra of OP (polyethylene glycol octylphenyl ether) emulsifier showed that it produced strong fluorescence at 304 nm. In pH 5.5 B-R buffer solution, the fluorescence intensity of OP emulsifier showed a good linear relationship with its concentration within a certain range. Based on this, a new high sensitivity determination method was established. The regression equation for the determination of the wavelength λex / λem = 226 nm / 304 nm was F = 22.12C + 9.825 (C: μg / mL, r = 0.9995) in the concentration range of 0.1-50.0 μg / mL with the detection limit of 0.068 μg / mL. The regression equation of the wavelength λex / λem = 276nm / 304nm was: F = 90.08C-10.12 (C: μg / mL, r = 0.9999). The detection limit was 0.053μg / mL. The determination of the same group of samples using these two sets of determination wavelength, the sample recovery rate of 96.5% or more, the test results were F test and t test analysis, drawn no significant difference between the two groups of data. The method has high sensitivity and strong anti-interference ability and can be used to determine the content of trace OP emulsifier in more complicated environmental samples.