目的探讨介入化疗对宫颈鳞癌细胞核形态、核仁组成区嗜银蛋白(AgNOR)的影响。方法用HE和嗜银染色对30例进行介入化疗前后的宫颈癌组织染色,并用真彩色图像分析系统测量肿瘤细胞的核面积、核周长、最大直径、AgNOR颗粒截面数、颗粒直径、颗粒总面积、颗粒总面积/核面积。结果介入后宫颈癌细胞的核面积、核周长、核最大直径较介入前缩小,P均<0.05,差异有显著性;介入前后核体积密度、核数密度、表面积密度比较P均<0.05,差异有显著性;介入前后核平均体积、核平均表面积、三维形状因子比较,P均<0.05,差异有显著性;介入后AgNOR颗粒截面数、颗粒总面积、颗粒直径、颗粒总面积/核面积较介入前减少或缩小,P均<0.05,差异有显著性。结论介入化疗可以缩小宫颈癌细胞的核面积、核周长,并且介入化疗前后宫颈癌的组织结构参数(VV、SV、NV,V、S、Rsv)存在显著差异;介入化疗可以减少AgNOR颗粒截面数,可以抑制宫颈癌细胞的增殖。 Objective To investigate the effects of interventional chemotherapy on nuclear morphology and nuclear argyrophilic protein (AgNOR) in cervical squamous cell carcinoma. Methods Cervical cancer tissues were stained with hematoxylin and eosin (HE) and argyrophilic silver staining before and after interventional chemotherapy, and the nuclear area, perinuclear length, maximum diameter, number of AgNOR particles, particle diameter, total particle size Area, total area of ​​particles / area of ​​nucleus Results After intervention, the nuclear area, nuclear perimeter and the maximum diameter of cervical cancer cells decreased compared with before intervention, both P <0.05, the difference was significant. Before and after intervention, nuclear bulk density, nucleus density and surface area density were all less than 0.05, The difference was significant; before and after intervention, the average volume of nucleus, the average surface area of ​​nucleus and three-dimensional shape factor, P <0.05, the difference was significant; the number of AgNOR particles cross section, total particle area, particle diameter, total particle area / Compared with before intervention to reduce or reduce, P <0.05, the difference was significant. Conclusion Interventional chemotherapy can reduce the nuclear area and perinuclear length of cervical cancer cells, and there are significant differences in the histological parameters (VV, SV, NV, V, S, Rsv) of cervical cancer before and after interventional chemotherapy. Interventional chemotherapy can reduce the cross section of AgNOR particles Number, can inhibit the proliferation of cervical cancer cells.