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目的检测清浆PSA与F-PSA,并探讨其在临床上的应作价值。方法 用化学发光标记免疫技术稀释检测法,检测30例健康男性组,30例BPH患者组和15例PCa患者组精浆PSA与F-PSA浓度水平,用回收试验和重复性试验和重复性试验来验证检测的正确性。并采用计算机SAS软件包(version 8.2)进行统计分析。结果(1)健康男性组精浆中PSA浓度范围为302800～1340000ng/ml,(587240±234394)ng/ml。F-PSA浓度范围为212800～778000ng/ml,(419720±148637)ng/ml。比血清PSA及F-PSA高出几十万倍。(2)BPH组精浆PSA与F-PSA浓度略高于正常对照组,但差异无统计学意义。(3)Pac组精浆PSA及F-PSA浓度低于正常对照组及BPH组,差异有高度显著性,有统计学意义。(4)前列腺癌患者精浆PSA与肿瘤分期Spearman和Kendall等级相关分析结果显示前列腺癌患者精浆PSA含量与肿瘤分期间相关不显著。结论 检测精浆PSA与F-PSA对前列腺癌与良性前列腺增生的鉴别有一定的临床应用价值。
Objective To detect serum PSA and F-PSA, and to explore its clinical value. Methods The serum PSA and F-PSA levels in 30 healthy men, 30 BPH patients and 15 PCa patients were measured by chemiluminescent immunoassay dilution assay. The recovery test, repetitive test and repeatability test To verify the correctness of the test. Statistical analysis was performed using a computer SAS package (version 8.2). Results (1) The concentration of PSA in healthy male seminal plasma ranged from 302800 ~ 1340000 ng / ml, (587240 ± 234394) ng / ml. F-PSA concentrations ranged from 212800 to 778000 ng / ml, (419720 ± 148637) ng / ml. Than several times higher than serum PSA and F-PSA. (2) The plasma PSA and F-PSA concentrations in BPH group were slightly higher than those in normal control group, but the difference was not statistically significant. (3) The concentrations of seminal plasma PSA and F-PSA in Pac group were lower than those in normal control group and BPH group, the difference was highly significant and statistically significant. (4) The results of Spearman and Kendall grade correlation analysis between seminal plasma PSA and tumor staging in prostate cancer patients showed that PSA level in seminal plasma of prostate cancer patients was not significantly correlated with tumor stage. Conclusion The detection of seminal plasma PSA and F-PSA in the differential diagnosis of benign prostatic hyperplasia and prostate cancer have some clinical value.