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目的分析2006-2007/06广州市大学城内不同校区的流感疫情的病原体。方法在疫区采集咽拭子标本,用MDCK细胞培养法分离病毒,用豚鼠红细胞凝集试验进行鉴别,对阳性标本进行病毒传代,然后用红细胞凝集抑制试验进行分型。提取不同校区病毒株的RNA,通过RT-PCR方法扩增流感病毒血凝素全长基因,测序并采用DNAs-tar软件对其核苷酸序列进行分析。结果2006年,35份咽拭子标本中有20份标本(57.1%)在HA试验中呈阳性反应,HI试验显示均为H1型流感病毒;2007年,48份咽拭子标本中有19份标本(39.6%)在HA试验中呈阳性反应,HI试验显示均为H3型流感病毒。2006年,两所校区的流感病毒株血凝素基因的同源性为96%;2007年,3所校区的流感病毒株血凝素基因的同源性为99%。结论2006年-2007年发生在不同校区的流感疫情都是各由同一病毒株所引起的。
Objective To analyze the pathogens of influenza outbreaks in different campuses in Guangzhou University City from 2006 to 2007/06. Methods Throat swab samples were collected in the affected area, and the virus was isolated by MDCK cell culture method. The positive cells were identified by the hemagglutination test in guinea pigs. The positive samples were passaged by virus and then typed by hemagglutination inhibition test. The RNA of different campus strains was extracted, and the full-length gene of influenza virus hemagglutinin was amplified by RT-PCR. The sequence of the gene was sequenced and its nucleotide sequence was analyzed by DNAs-tar software. Results In 2006, 20 of the 35 throat swab specimens (57.1%) were positive in the HA test, and all of the HI tests showed H1 virus. In 2007, 19 of the 48 throat swab specimens Specimens (39.6%) were positive in the HA test, and the HI test showed that all were H3 viruses. In 2006, the homology of the hemagglutinin gene of the influenza virus strain in two campuses was 96%. In 2007, the homology of the hemagglutinin gene of the influenza virus strains in the three campuses was 99%. Conclusions Influenza outbreaks in different campuses in 2006-2007 were caused by the same strain of virus.