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目的对粤东地区的自然人群、动物宿主和传播媒介进行调查研究，以证实该地区莱姆病疫源地的存在。方法选取粤东梅州市的梅县梅南林场、大埔县丰溪林场和平远县水溪管理区为调查点，用间接免疫荧光抗体试验（IFA）和间接酶联免疫吸附试验（ELISA）进行人群和宿主动物的血清学调查，个案流行病学调查，动物宿主和媒介生物的病原学调查。结果从检测2184份自然人群的血清得知，该地区人群莱姆病感染率为10．30％，林区（10．37％）与非林区（10．27％）人群感染率差异无显著性（X2＝0．21，P＞0．5）。检测牛和野鼠类动物血清各30份，感染率分别为23．33％和43．33％，并从褐家鼠和白腹巨鼠分离到2株莱姆病螺旋体，因此，我们认为鼠类和牛可能是当地莱姆病螺旋体的重要动物宿主。检查蜱506只，台湾角血蜱为优势种（97．43％），台湾角血蜱中肠带莱姆病螺旋位率为16．00％（8／50），并分离出1株莱姆病螺旋体，可见台湾角血蜱是当地莱姆病螺旋体主要生物媒介。结果首次确认粤东地区为我国莱姆病的一个自然疫源地。
Objective To investigate the natural populations, animal hosts and the media in eastern Guangdong to confirm the existence of Lyme disease in this area. Methods Meinlin Forest Farm in Meixian, Meizhou, Fengxi Forest Farm in Dapu County and Shixi Management District in Pingyuan County were selected as the investigation points. The indirect immunofluorescence antibody test (IFA) and indirect enzyme-linked immunosorbent assay (ELISA) And serological surveys of host animals, case-by-case epidemiological investigations, etiological investigations of animal hosts and vector organisms. Results From the sera from 2184 natural persons, the prevalence of Lyme disease in the population was 10.30%, while there was no significant difference in the infection rates among the forest areas (10.37%) and non-forest areas (10.27%) (X2 = 0.21, P> 0.5). Thirty 30 serum samples of cattle and vole were detected, the infection rates were 23.33% and 43.33% respectively. Two strains of Borrelia burgdorferi were isolated from Rattus norvegicus and Pulsatilla gigas. Therefore, And cattle may be important animal hosts for local Lyme disease spirochetes. 506 ticks were examined, the dominant species of H. japonicus was 97.43%, the percentage of Lyme disease was 16.00% (8/50) in H. gondii, and 1 strain of Lyme Spirochetes, we can see that the black hornworm is a major biological mediator of local Lyme disease. The results confirmed for the first time that eastern Guangdong is a natural foci of Lyme disease in our country.