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A biophotochromic material, i.e., genetic mutant bacteriorhodopsin (BR-D96N), was experimentally studied on its holographic recording properties. The saturation absorption curve and the diffraction efficiency curve were measured respectively. As holographic storage application, reflection type polarization holograms were recorded on the BR-D96N film. The 173° configuration between the object and reference beams proves that the spatial resolution of the film is over 6000 lines/mm. By using phase conjugate wave of reference beam as reconstruction beam, the distorted object image introduced by the optical components and the defects in the recording medium can be well corrected.