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目的:观察清肝活血方对酒精性肝病大鼠乙醇代谢关键酶基因表达的影响。方法:用乙醇、玉米油、吡唑等制备酒精性肝病大鼠模型。RT-PCR法检测大鼠肝组织ADH及CYPⅡElmRNA表达。结果:酒粉性肝病大鼠模型表现为肝功能异常,以AST变化为显著;模型组ADH活性及其mRNA与CYPⅡE1 mRNA表达下降;高剂量清肝活血方组大鼠ADH活性及ADH与CYPⅡE1 mRNA的表达显著提高。结论:清肝活血方能显著提高模型组大鼠ADH活性及ADH与YPⅡE1mRNA的表达,促进肝脏的解毒功能。
Objective: To observe the effect of qinggan huoxue decoction on alcohol metabolism key enzyme gene expression in alcoholic liver disease rats. Methods: Alcoholic liver disease rat model was prepared with ethanol, corn oil and pyrazole. The expression of ADH and CYPⅡElmRNA in rat liver tissue were detected by RT-PCR. Results: The expression of ADH activity and mRNA and CYPⅡE1 mRNA in model rats were significantly different from those in rats with alcoholic liver disease. The ADH activity and ADH and CYPⅡE1 mRNA expression of rats in high dose Qinggan Huoxue recipe The expression was significantly increased. Conclusion: Qinggan Huoxue Decoction can significantly improve ADH activity and expression of ADH and YPⅡE1 mRNA in rats in model group, and promote liver detoxification.