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目的:观察CK19在口腔黏膜癌变各个阶段的表达,探寻其与口腔黏膜癌变之间的关系及意义。方法:4NQO诱导SD大鼠的口腔黏膜癌变,运用免疫组织化学的方法检测CK19在口腔黏膜癌变过程中各阶段的动态变化,分析CK19与口腔黏膜癌变的关系。结果:在大鼠正常舌黏膜组织中,CK19阳性染色的细胞散在分布于黏膜基底层,基底上层不表达;随着大鼠舌黏膜上皮异常增生程度的增加,CK19表达于黏膜基底上层;随着病变程度加重,CK19表达强度增强,阳性率增加,差异具有统计学意义。结论:4NQO诱发大鼠舌黏膜癌变过程中,CK19蛋白表达水平随病变程度加重显著升高,提示CK19与口腔上皮细胞的癌变有关;CK19的异常表达可作为早期诊断口腔鳞状细胞癌的辅助指标之一。
OBJECTIVE: To observe the expression of CK19 in various stages of oral mucosal carcinogenesis and to explore its relationship with the carcinogenesis of oral mucosa. Methods: 4NQO induced oral mucosal carcinogenesis in SD rats. Immunohistochemistry was used to detect the dynamic changes of CK19 in various stages of oral mucosa carcinogenesis. The relationship between CK19 and oral mucosal carcinogenesis was analyzed. Results: In normal rat tongue mucosa tissue, CK19-positive staining cells scattered in the basal mucosa, the basal layer is not expressed; with the tongue mucosal epithelial dysplasia increased, CK19 expression in the mucosal basal layer; with The degree of lesion increased, CK19 expression intensity increased, the positive rate increased, the difference was statistically significant. CONCLUSION: CK19 protein expression is significantly increased with the severity of pathological changes induced by 4NQO in rat tongue carcinomas, suggesting that CK19 may be associated with carcinogenesis of oral epithelial cells. Aberrant expression of CK19 may serve as an adjunct to early diagnosis of oral squamous cell carcinoma one.