目的　在大肠杆菌中表达柯萨奇 B1(CVB1)病毒 VP1蛋白。方法　用限制性酶切方法将 CVB1VP1基因从 p MD18-T-VP1上切下 ,连接至 p QE3 0构建原核表达系统 p QE3 0 -VP1。经酶切和 PCR验证后转化至大肠杆菌 BL2 1(DE3 ) ,用 IPTG诱导目的基因表达。SDS-聚丙烯酰胺凝胶电泳和 Western-blot检测 VP1蛋白的表达。结果　酶切、PCR证明构建的原核表达系统携带方向正确的 CVB1VP1基因。SDS-PAGE和 Western-blot实验均可于 3 3 .0 k Da处见到目的条带。结论　 CVB1VP1基因在大肠杆菌的成功表达为开发新的血清学检测试剂提供了基础 Objective To express Coxsackievirus B1 (CVB1) virus VP1 protein in E. coli. Methods The CVB1VP1 gene was excised from pMD18-T-VP1 by restriction enzyme digestion and ligated into pQE3 0 to construct prokaryotic expression system pQE3 0 -VP1. The recombinant plasmid was transformed into E.coli BL21 (DE3) after digestion and PCR. The target gene was induced by IPTG. SDS-polyacrylamide gel electrophoresis and Western-blot detection of VP1 protein expression. Results The results of enzyme digestion and PCR proved that the constructed prokaryotic expression system carried the correct CVB1VP1 gene. SDS-PAGE and Western-blot experiments can be seen at 3300 k Da at the destination band. Conclusions The successful expression of CVB1 VP1 gene in E. coli provides the basis for developing new serological test reagents