论文部分内容阅读
目的:观察葱白提取物纳米乳口腔喷雾剂对急性心肌缺血兔心电图及心肌梗死(简称心梗)面积的影响。方法:日本大耳白兔56只,随机分为假手术组、空白模型组、硝酸甘油组、葱白提取物组、空白纳米组、葱白纳米乳组和葱白纳米乳原位凝胶组,每组8只。术后及时舌下给药,硝酸甘油组、葱白提取物组、空白纳米组、葱白纳米乳组和葱白纳米乳原位凝胶组给予相应的制剂5喷,假手术组和空白模型组给予同体积的生理盐水。记录给药前及用药后0、1、5、10、15、30、60、120和180min的心电图,实验结束后处死动物,计算心梗面积。结果:①与模型组比较,各治疗组舌下给药后均可缩小兔冠脉结扎所致心肌梗死范围(P<0.05),葱白提取物及葱白提取物纳米制剂组减小心肌梗死面积的程度与硝酸甘油相当(P>0.05),葱白提取物纳米乳组和其纳米乳原位凝胶组与葱白提取物组未见明显差异(P>0.05);②对心电图的影响:与模型组比较,舌下给药后不同时间点各治疗组舌下给药后均可以使ST段显著降低(P<0.05);给药后1～5min,葱白提取物纳米乳组与硝酸甘油组比较作用相当(P>0.05),优于葱白提取物组(P<0.05)。葱白纳米乳原位凝胶起效时间为10～30min,葱白提取物起效时间为5～10min,葱白纳米乳组起效时间为0～5min,葱白纳米乳优于葱白提取物和葱白纳米乳原位凝胶,与硝酸甘油起效时间相当。结论:葱白提取物纳米乳口腔喷雾剂对ST段和心肌梗死面积的作用优于葱白提取物和葱白纳米乳原位凝胶,与硝酸甘油起效时间相当。
OBJECTIVE: To observe the effects of the extract of Nanqianbai extract on the area of electrocardiogram and myocardial infarction (MI) in acute myocardial ischemia rabbits. METHODS: Fifty-six Japanese white rabbits were randomly divided into sham operation group, blank model group, nitroglycerin group, light blue extract group, blank nano group, light blue nano milk group and light blue nano milk in situ gel group. 8 only. Immediately after sublingual administration, the nitroglycerin group, the light blue extract group, the blank nano group, the light blue nano milk group, and the light blue nanoemulsion in situ gel group were given the corresponding formulation 5 sprays, the sham group and the blank model group were given the same The volume of saline. Electrocardiograms before and after administration were recorded at 0, 1, 5, 10, 15, 30, 60, 120 and 180 minutes. Animals were sacrificed after the end of the experiment to calculate the area of the myocardial infarction. Results:1Compared with the model group, the myocardial infarct size caused by coronary artery ligation in rabbits could be reduced after sublingual administration in each treatment group (P<0.05). The concentration of myocardial infarct was reduced by the onion white extract and the onion white extract nano preparations group. The degree was equivalent to that of nitroglycerin (P>0.05). There was no significant difference between the onion white extract nanoemulsion group and its nanoemulsion in situ gel group and onion extract group (P>0.05); 2Effect on electrocardiogram: model group Compared with sublingual administration, sublingual administration of each sublingual administration group resulted in a significant decrease in ST segment (P<0.05); 1 to 5 min after administration, the comparison between the onion white extract nanoemulsion group and the nitroglycerin group was observed. Quite (P>0.05), better than the light blue extract group (P<0.05). The onset time of in situ white nanoemulsion gel is 10-30min, the onset time of onion white extract is 5～10min, and the onset time of onion white nanoemulsion is 0-5min. The onion white nanoemulsion is better than the onion white extract and the onion white nanoemulsion In-situ gel, comparable to nitroglycerin onset time. CONCLUSIONS: The effect of the onion-inhalation extract of nanoemulsion on the infarct size of ST segment and myocardial is better than that of the onion white extract and the onion white nanoemulsion in situ gel, which is equivalent to the onset time of nitroglycerin.