哺乳和应激等刺激既能促进催产素释放,又可抑制黄体生成素分泌,提示这些神经内分泌反应之间存在着功能上的相互作用。已知产生并释放催产素和促性腺素释放激素的神经元均位于下丘脑,其末梢终止于正中隆起的外侧带。Gambacciani 等推测,催产素可能通过抑制下丘脑促性腺素释放激素的释放进而抑制黄体生成素分泌,为此,他们采用了离体脑组织孵化技术,检验了催产素对内侧基底部下丘脑或正中隆起释放促性腺素释放激素的影响。实验用雄性大鼠,断头后取其正中隆起或内侧基底部下丘脑(包括弓状核、腹内侧核和ME),在通氧、 Suction and stress stimulation not only promote the release of oxytocin, but also inhibit the secretion of luteinizing hormone, suggesting that there is a functional interaction between these neuroendocrine responses. Neurons that produce and release both oxytocin and gonadotropin-releasing hormone are known to be located in the hypothalamus, with their distal termini terminating in the lateral mid-bulge. Gambacciani et al hypothesized that oxytocin may inhibit the release of hypothalamic gonadotropin-releasing hormone and thus inhibit the secretion of luteinizing hormone, for which they used isolated brain tissue incubation technique to test the oxytocin on the medial base of the hypothalamus or median elevation Release of gonadotropin-releasing hormone. Experimental male rats, after decapitation from the median elevation or medial basal hypothalamus (including arcuate nucleus, ventral medial nucleus and ME), in the oxygen,