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目的 通过观察转化生长因子 β1 (TGF β1 )在口腔癌中的表达情况 ,探讨TGF β1 与口腔癌发生发展的关系 ,为利用TGF β1 治疗口腔癌患者提供理论和实验依据。方法 本实验收集口腔鳞癌根治标本 5 8例和 1 0例正常口腔粘膜做对照 ,按照分化程度、浸润方式、有无淋巴结转移进行分组 ,采用免疫组化SABC法对口腔癌中的TGF β1 抗原进行检测。结果 正常口腔粘膜和 5 8例口腔鳞癌标本均表达TGF β1 。但在口腔鳞癌标本中TGF β1 主要在癌细胞周围基质中表达 ,而癌细胞本身不表达TGF β1 。在癌基质中强表达TGF β1 的肿瘤组织分化程度低 ,浸润能力强 ,淋巴结转移率高。结论 口腔癌细胞本身自分泌TGF β1 的能力减弱或消失 ;而肿瘤基质中旁分泌产生的TGF β1 促进了肿瘤的浸润、转移
Objective To observe the expression of transforming growth factor-β1 (TGF-β1) in oral cancer and to explore the relationship between TGF-β1 and the occurrence and development of oral cancer, and to provide theoretical and experimental basis for the treatment of oral cancer with TGF-β1. Methods Fifty-eight cases of oral squamous cell carcinoma and 10 cases of normal oral mucosa were collected and divided into groups according to the degree of differentiation, infiltration and lymph node metastasis. Immunohistochemical SABC method was used to detect the expression of TGFβ1 antigen in oral cancer Test. Results Normal oral mucosa and 58 cases of oral squamous cell carcinoma specimens expressed TGFβ1. In oral squamous cell carcinoma, however, TGFβ1 is mainly expressed in the surrounding matrix of cancer cells, whereas the cancer cells themselves do not express TGFβ1. The strong expression of TGFβ1 in cancer stroma tumor tissue differentiation is low, invasive ability, lymph node metastasis rate. Conclusions The ability of oral cancer cells to self-secrete TGFβ1 is weakened or disappeared. However, paracrine TGFβ1 promotes tumor invasion and metastasis