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目的检测呼吸道感染患儿肺炎支原体抗体、淋巴细胞亚群,分析肺炎支原体肺炎患儿免疫学变化。方法收集2006年6月～2007年11月苏州地区住院2563份血清儿童急性下呼吸道感染患儿应用ELISA进行肺炎支原体(MP)抗体定量检测;用流式细胞仪对其中30例(年龄1个月～36个月)MP肺炎患儿外周血进行CD3+、CD4+、CD8+、CD19+、NK(CD16+56)+及CD23+指标测定,设同龄健康对照组20例。结果 2563例标本,MP阳性557例(21.74%)。MP感染3～岁组阳性检出率明显高于小于3岁组(x2=167.087,P<0.01);女性儿童MP阳性率高于男性儿童(x2=45.047,P<0.01)。MP肺炎患儿CD3+、CD4+、NK(CD16+56)+细胞百分率低于正常对照组(t=-2.207、P<0.05,t=-2.505、P<0.05,t=-7.137,P<0.01),CD19+细胞百分率高于正常对照组(t=3.832,P<0.01);CD8+和CD23+细胞百分率无明显差异(t=-1.491、P>0.05,t=1.915、P>0.05)。结论苏州地区MP感染3～岁儿童多见,MP肺炎患儿细胞免功能有不同程度下降。
Objective To detect Mycoplasma pneumoniae antibodies and lymphocyte subsets in children with respiratory tract infection and analyze the immunological changes in children with Mycoplasma pneumoniae pneumonia. Methods A total of 2563 children with acute lower respiratory tract infection in children with acute respiratory tract infection from June 2006 to November 2007 in Suzhou were enrolled in this study. Quantitative detection of Mycoplasma pneumoniae (MP) antibody was performed by ELISA. Flow cytometry was used to detect 30 cases (age 1 month ~ 36 months). Peripheral blood of children with MP pneumonia were measured for CD3 +, CD4 +, CD8 +, CD19 +, NK (CD16 + 56) + and CD23 +, and 20 healthy controls of the same age were enrolled. Results 2563 cases of specimens, MP positive 557 cases (21.74%). The positive rate of MP infection in 3 ~ age group was significantly higher than that of less than 3 years old group (x2 = 167.087, P <0.01). The positive rate of MP in female children was higher than that in male children (x2 = 45.047, P <0.01). The percentage of CD3 +, CD4 +, NK (CD16 + 56) + cells in children with MP pneumonia was lower than that in the normal control group (t = -2.207, P <0.05, t = -2.505, , While the percentage of CD19 + cells was higher than that of the normal control group (t = 3.832, P <0.01). The percentage of CD8 + and CD23 + cells had no significant difference (t = -1.491, P> 0.05, t = 1.915, P> 0.05). Conclusion MP infection in Suzhou is more common in children aged 3 to 4 years. The immune function of children with MP pneumonia decreased to varying degrees.