论文部分内容阅读
该试验以无核白葡萄的愈伤组织为试验材料,MS+0.50 mg/L6-BA+0.5 g/mLCH为培养基,分别研究了不同浓度的2,4-D和NAA对葡萄愈伤组织诱导成苗的影响,二者浓度均设置为0.00、0.50、0.80、1.10、1.40、1.70、2.00 mg/L。结果表明:2,4-D对葡萄愈伤组织诱导成苗的影响比较明显,浓度较低时对其有促进作用。随着浓度的升高,当浓度达到一定值时,则对其具有抑制作用,当2,4-D的浓度为1.40mg/L时,诱导成苗后的葡萄愈伤组织的成苗率最高,达到7%;而NAA对葡萄愈伤组织诱导成苗的影响没有2,4-D明显,当NAA浓度为0.80mg/L时,诱导成苗后葡萄愈伤组织的成苗率最高为4%。
In this experiment, the callus of non-nuclear white grape was used as the experimental material and MS + 0.50 mg / L 6-BA + 0.5 g / m LCH was used as medium to study the effect of 2,4-D and NAA in different concentrations on callus The effects of inducing seedlings were both set at 0.00,0.50,0.80,1.10,1.40,1.70,2.00 mg / L. The results showed that the effect of 2,4-D on the callus induction of grafted callus was obvious. When the concentration was low, the effect of 2,4-D was promoted. With the increase of concentration, when the concentration reached a certain value, it was inhibited. When the concentration of 2,4-D was 1.40mg / L, the seedling rate of callus was the highest , Which reached 7%. However, the effect of NAA on callus induction was not obvious when the NAA concentration was 0.80mg / L. The highest seedling rate of callus was 4 %.