目的研究茶碱与胰蛋白酶之间的相互作用机制。方法采用荧光光谱法和紫外光谱法,研究作用体系的光谱变化。结果茶碱与胰蛋白酶作用体系的ΔrHm为6.302 k J/mol,ΔrSm为130.6 J/K·mol,结合距离r为1.70 nm。结论茶碱对胰蛋白酶的荧光强度具有猝灭作用,二者之间主要是疏水作用力,且相互之间存在能量转移,茶碱可改变胰蛋白酶的构象。 Objective To study the interaction between theophylline and trypsin. Methods Fluorescence spectroscopy and UV spectroscopy were used to study the spectral changes of the system. Results The ΔrHm of theophylline and trypsin system was 6.302 kJ / mol, ΔrSm was 130.6 J / K · mol, and the binding distance r was 1.70 nm. Conclusion Theophylline has a quenching effect on the fluorescence intensity of trypsin. The interaction between the two is mainly hydrophobic, and there is energy transfer between them. Theophylline can change the conformation of trypsin.