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目的:观察实验性溃疡性结肠炎大鼠外周血Th17细胞的变化及黄芪多糖对其的影响。方法:雄性清洁级Wistar大鼠40只,随机分成4组:空白组、模型组、黄芪多糖组、柳氮磺胺砒啶(SASP)组。各组在造模后第3d开始给药,共14 d。给药结束后,腹主动脉取血,离心取血清,ELISA法测定IL-17、TGF-β和IL-23。取距肛门2-10cm处结肠,沿肠黏膜缘剪开肠腔,取病变处组织,进行HE染色。结果:与空白组相比,模型组大鼠血清中IL-17、IL-23、TGF-β含量显著增高(P<0.05)。与模型组比较,SASP组与黄芪多糖组大鼠血清中IL-17、IL-23、TGF-β含量显著降低(P<0.05)。与SASP组比较,黄芪多糖组大鼠血清中IL-17、IL-23、TGF-β含量降低(P<0.05)。结肠病理显示:在各治疗组中,黄芪多糖组黏膜结构最完整、炎症细胞最少。结论:Th17细胞在实验性溃疡性结肠炎大鼠外周血中明显升高,黄芪多糖可能通过调节大鼠血清中IL-23、TGF-β的表达来调节Th17细胞,以减轻肠黏膜炎症损伤。
Objective: To observe the changes of Th17 cells in experimental ulcerative colitis rats and the effect of astragalus polysaccharides. Methods: Forty male Wistar rats were randomly divided into 4 groups: blank group, model group, Astragalus polysaccharide group and sulfasalazine (SASP) group. Each group began administration on the 3rd day after modeling, a total of 14 days. After administration, blood was taken from the abdominal aorta and serum was collected by centrifugation. IL-17, TGF-β and IL-23 were measured by ELISA. Take the colon 2-10cm away from the anus, cut the intestine along the intestinal mucosal margin, take lesions at the organization, HE staining. Results: Compared with the blank group, the contents of IL-17, IL-23 and TGF-β in the model group were significantly increased (P <0.05). Compared with model group, the levels of IL-17, IL-23 and TGF-β in serum of SASP group and Astragalus polysaccharide group were significantly decreased (P <0.05). Compared with SASP group, the content of IL-17, IL-23 and TGF-β in astragalus polysaccharide group decreased (P <0.05). Colon pathology showed that in the treatment groups, the mucosal structure of Astragalus polysaccharide group was the most complete with the least number of inflammatory cells. Conclusion: Th17 cells are significantly increased in the peripheral blood of experimental ulcerative colitis rats. Astragalus polysaccharide may regulate Th17 cells through regulating the expression of IL-23 and TGF-β in the serum of rats to relieve the inflammatory damage of intestinal mucosa.