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Objective:Hyperglycosylated human chorionic gonadotropin(hCG) is a variant of hCG made by cytotrophoblast cell.Here we examine the role of hyperglycosylated hCG in placenta growth and invasion.Methods:JEG-3 choriocarcinoma cells and term cytotrophoblast monolayer culture were prepared.The effect of supplemental hyperglycosylated hCG and hCG was investigated. Growth of these cells was examined by increase incell number.Invasion was investigated using Matrigel basement membrane cells.The proportion of cell invadingMatrigel was determined. Results:Term cytotrophoblast cell andJEG-3 choriocarcinoma cells grew to5427±834 cells (109%) and7114±553 cells(142%).With the supplementation of hyperglycosylated hCG, they grew significantly wider to7633±177 cells(142%) and10315±1477 cells(206%).With the supplementation of hCG they diminished to4227±769 cells(78%) and5620±657 cells(79%).Term cytotrophoblast cell andJEG-3 choriocarcinoma cells penetratedMatigel membranes to(40.0±10.0)% and(46.0±9.8)%.Hyperglycosylated hCG significantly enhanced penetration to(76.0±13.0)% and(84.0±6.6)%. hCG diminished penetration to(32.0±9.1)% and(32.0±4.5)%.Conclusions:Hyperglycosylated hCG enhances both cytotrophoblast growth and cytotrophoblast cell invasion. hCG minimally suppresses growth and invasion.