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目的:探讨核转录因子-κB亚单位p50(NF-κBp50)在季节性变应性鼻黏膜中的表达及活性状态。方法:采用免疫组织化学方法检测16例季节性变应性鼻炎(SAR组)下鼻甲黏膜组织中NF-κBp50的表达,其中6例为非发作期患者,10例为发作期患者;并与10例鼻中隔偏曲患者下鼻甲黏膜进行对照。采用电泳迁移率滞后分析(EMSA)检测鼻黏膜组织中核蛋白提取物与特异性NF-κB探针的结合活性。结果:NF-κBp50在SAR组及对照组中均有表达,p50阳性表达主要分布在2组鼻黏膜的上皮细胞、炎性细胞、腺上皮细胞及血管内皮细胞的胞质和部分胞核中;SAR组p50胞核阳性染色的阳性细胞百分率发作期是(41.83±4.43)%,非发作期是(37.19±3.93)%,两者比较差异无统计学意义(P>0.05)。对照组p50胞核阳性染色的阳性细胞百分率仅(8.89±1.32)%。SAR组(包括发作期和非发作期)与对照组p50胞核阳性染色的阳性细胞百分率间比较差异有统计学意义(P<0.01);SAR组NF-κB的DNA结合的32P标记电泳带密度扫描值为32.14±8.73,与对照组(11.12±3.42)比较,差异有统计学意义(P<0.05)。结论:在对照组鼻黏膜中保持低水平的表达,在SAR鼻黏膜中表达显著增高,且其与DNA-蛋白结合活性增高,提示NF-κBp50可能参与维持鼻黏膜的生理功能,并在SAR鼻黏膜持续慢性炎症反应中起重要作用。
Objective: To investigate the expression and activity of nuclear transcription factor-κB subunit p50 (NF-κBp50) in seasonal allergic nasal mucosa. Methods: Immunohistochemistry was used to detect the expression of NF-κB p50 in the nasal mucosa of 16 patients with seasonal allergic rhinitis (SAR group), of which 6 were non-episode patients and 10 were in the attack stage. Cases of nasal septum deviation inferior turbinate mucosa for comparison. Electrophoretic mobility shift assay (EMSA) was used to detect the binding activity of nuclear protein extract to specific NF-κB probe in nasal mucosa. Results: NF-κBp50 was expressed in both SAR and control groups. The expression of p50 was mainly located in the cytoplasm and part of the nucleus of epithelial cells, inflammatory cells, glandular epithelial cells and vascular endothelial cells in two groups of nasal mucosa. The percentage of positive cells in p50 nucleus positive staining in SAR group was (41.83 ± 4.43)% in non-attack period and (37.19 ± 3.93)% in non-attack period. There was no significant difference between the two groups (P> 0.05). The positive rate of p50 nucleus positive staining in control group was only (8.89 ± 1.32)%. There were significant differences in the percentages of positive cells in p50 nucleus in SAR group (P <0.01). The DNA-bound 32P-labeled electrophoretic bands in SAR group The scan value was 32.14 ± 8.73, which was significantly different from that of the control group (11.12 ± 3.42) (P <0.05). CONCLUSIONS: The low expression level in the nasal mucosa of the control group is significantly increased in SAR nasal mucosa, and its binding activity with DNA-protein is increased, suggesting that NF-κBp50 may be involved in maintaining the physiological function of the nasal mucosa and in SAR nasal mucosa Mucosal sustained chronic inflammatory response plays an important role.