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目的:通过卵巢癌HO-8910细胞表面的TfR(CD71)在rhEPO干预前后的表达情况来观察rhEPO对细胞增殖的影响,探讨其在肿瘤相关性贫血中的治疗价值。方法:用RT-PCR法检测经不同浓度的rhEPO处理后的和未经rhEPO处理的HO-8910细胞表面的TfR mRNA表达情况:用流式细胞仪观察和检测处理前后的HO-8910细胞周期上的差异。结果:不同浓度的rhEPO培养HO-8910细胞48 h,72 h后,TfR表达均减少,与同期对照组相比差异有显著意义(P<0.01);经不同浓度的EPO在不同时间处理后的HO-8910及未经EPO处理的HO-8910在细胞周期上,与同期对照组相比差异有显著意义(P<0.01),且较对照组有所减少。结论:经过高浓度的rhEPO处理后,卵巢癌HO-8910细胞表面TfR的表达减少,S期细胞百分率也有所降低,间接提示了高浓度的rhEPO可能通过铁代谢抑制卵巢癌HO-8910细胞的生长、增殖。
OBJECTIVE: To observe the effect of rhEPO on cell proliferation by TfR (CD71) on the surface of ovarian cancer HO-8910 cells before and after rhEPO intervention, and to explore its therapeutic value in tumor-associated anemia. Methods: The expression of TfR mRNA on HO-8910 cells treated with different concentrations of rhEPO and without rhEPO was detected by RT-PCR: The cell cycle of HO-8910 cells was observed by flow cytometry The difference. Results: After treated with different concentrations of rhEPO, the expression of TfR in HO-8910 cells after 48 h and 72 h decreased significantly compared with that in the control group (P <0.01). After treated with different concentrations of EPO for different time HO-8910 and HO-8910 without EPO had significant difference (P <0.01) in the cell cycle compared with the control group at the same period, and decreased compared with the control group. CONCLUSION: After treated with high concentration of rhEPO, the expression of TfR on the surface of HO-8910 cells decreased and the percentage of S phase cells decreased, suggesting that high concentrations of rhEPO could inhibit the growth of HO-8910 cells via iron metabolism ,proliferation.