论文部分内容阅读
目的:观察启明丸对白内障模型大鼠MDA、SOD、GSH-Px、G-6-PD水平及晶状体蛋白含量变化的影响。方法:将50只SD大鼠按照随机数字表分为5组,对照组,模型组及启明丸低、中、高剂量组;采用亚硒酸钠造模,造模成功后予相应药物灌胃,灌胃28 d后,摘取大鼠双侧眼球晶状体,匀浆,离心,分离上清液,测定MAD、SOD、GSH-Px和G-6-PD水平及晶状体匀浆蛋白含量。结果:启明丸中、高剂量组MDA及晶状体匀浆蛋白含量均低于模型组,SOD、GSH-Px、G-6-PD水平均高于模型组(P<0.05);启明丸中、高剂量组与低剂量组比较,差异均有统计学意义(P<0.05)。启明丸中、高剂量均能够调整MDA、SOD、GSH-Px、G-6-PD水平,降低晶状体中蛋白含量。结论:启明丸能调整白内障模型大鼠晶状体MDA、SOD、GSH-Px、G-6-PD水平,降低晶状体中蛋白含量,从而改善晶状体混浊程度。
Objective: To observe the effect of Qiumingwan on the levels of MDA, SOD, GSH-Px, G-6-PD and the content of lens protein in cataract model rats. Methods: Fifty SD rats were randomly divided into five groups: control group, model group and Qiming Mian low, medium and high dose groups. Sodium selenite was used to make model. After intragastric administration for 28 days, the bilateral ocular lens of rats were removed, homogenized and centrifuged. The supernatant was separated and the level of MAD, SOD, GSH-Px and G-6-PD and the content of lens homogenate were measured. Results: The contents of MDA and lens homogenate in medium and high dose Qiuming Pills were lower than those in model group, and the levels of SOD, GSH-Px and G-6-PD were higher than those in model group (P <0.05) Compared with the low dose group, the differences were statistically significant (P <0.05). In Qiming Pill, medium and high dose of MDA could regulate the levels of MDA, SOD, GSH-Px and G-6-PD, and reduce the protein content in the lens. Conclusion: Qiming Pill can adjust the level of MDA, SOD, GSH-Px, G-6-PD in the lens of cataract model rats and decrease the protein content in the lens so as to improve the lens opacity.