采用响应面法优化桦褐孔菌水提液多糖及超氧化物歧化酶的提取工艺条件.在单因素试验基础上,应用Box-Benhnken中心组合法进行3因素3水平试验设计,以水提液多糖含量、SOD活性为双响应值,进行响应面分析.结果表明,最佳提取工艺条件为提取时间3.4h,提取温度83℃,料液比为1∶36.在此条件下,经四次重复性实验验证,水提液中多糖含量均值为3.41mg·mL-1,SOD活性均值为5085 U·mL-1.“,”Response surface methodology (RSM) was employed to optimize the extraction conditions of Inonotus obliquus polysaccharides with superoxide dismutase (SOD).Based on single-factor tests,Box-Benhnken center composite experiment was carried out with three factors.RSM was used to determine the effect of prime factors on the polysaccharides content and SOD activity of Inonotus obliquus aqueous extract.Results showed that the optimum extraction conditions by RSM were as follows:extracting time of 3.4 h,extracting temprature of 83℃ and the ratio of water to material of 1∶36.Following repeated experiments four times,the average polysaccharides content and SOD activity was 3.41mg·mL-1 and 5085U·mL-1,respectively.