论文部分内容阅读
目的:探讨丹参对内毒素(LPS)诱导的小鼠急性肺损伤(ALI)保护作用及其机制。方法:通过腹腔注射大肠杆菌LPS(5ml/kg)诱导小鼠ALI。30只雄性C57小鼠随机均分为正常组、模型组和丹参组,每组各10只。丹参组于LPS注射前1h腹腔注射丹参(10mg/kg),模型组和正常组注射等量生理盐水。LPS注射后6h,观察各组血气、肺组织病理形态、肺湿/干比(W/D),支气管肺泡灌洗液、肺组织和血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)的表达,以及IκB-α、TLR-4、MyD88、p-p65和p65蛋白表达。结果:丹参能有效降低LPS所致肺组织血气和病理学改变,减少肺W/D以及TNF-α、IL-1β、IL-6、TLR-4、MyD88和p-p65的表达,增加IκB-α的表达。结论:丹参对ALI小鼠有保护作用,其作用机制与抑制TLR-4/NF-κB介导的炎症相关。
Objective: To explore the protective effect of Salvia miltiorrhiza on endotoxin (LPS) -induced acute lung injury (ALI) in mice and its mechanism. Methods: Mouse ALI was induced by intraperitoneal injection of E. coli LPS (5ml / kg). Thirty male C57 mice were randomly divided into normal group, model group and Salvia miltiorrhiza group, 10 in each group. Salvia miltiorrhiza group was intraperitoneally injected with Salvia miltiorrhiza (10mg / kg) 1h before LPS injection, and normal saline was injected into the model group and the normal group. Six hours after LPS injection, blood gas, pathological changes of lung tissue, W / D, bronchoalveolar lavage fluid, tumor necrosis factor alpha (TNF-α) and interleukin IL-1β, IL-6 and IκB-α, TLR-4, MyD88, p-p65 and p65 protein expression. Results: Salvia miltiorrhiza can effectively reduce the blood gas and pathological changes of lung tissue induced by LPS, reduce the expression of W / D and TNF-α, IL-1β, IL-6, TLR-4, MyD88 and p-p65, α expression. Conclusion: Salvia miltiorrhiza has a protective effect on ALI mice and its mechanism is related to the inhibition of TLR-4 / NF-κB-mediated inflammation.