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恶性黑色素瘤是一种恶性度很高的肿瘤.肿瘤浸润淋巴细胞(TIL)是迄今为止恶黑治疗的最为有效的免疫活性细胞.然而,TIL细胞的应用必须同时以大量的白细胞介素2(IL-2)以维持其活性.而IL-2在体内的半衰期很短,用量大、代价昂贵,而且能够引起一系列的毒副作用.如果将IL-2基因插入TIL细胞、使其一方面释放IL-2维持自身的活性,另一方面使TIL细胞能携带IL-2基因到肿瘤部位释放具有广泛免疫活性的IL-2,一定能使TIL细胞的抗肿瘤作用如虎添翼.我们以逆转录载体成功地将IL-2基因导入恶黑的TIL细胞中,并得以持续、稳定的表达.IL-2基因的PCR扩增证实外源性IL-2基因成功插入TIL细胞中,转染率在1‰～
Malignant melanoma is a highly malignant tumor. Tumor infiltrating lymphocytes (TIL) are by far the most effective immunocompetent cells for ebony treatment. However, the application of TIL cells must simultaneously involve a large amount of interleukin 2 ( IL-2) to maintain its activity. The half-life of IL-2 in vivo is very short, large amount, expensive, and can cause a series of toxic side effects. If IL-2 gene is inserted into the TIL cells to release on the one hand IL-2 maintains its own activity, on the other hand, enables TIL cells to carry the IL-2 gene to the tumor site to release IL-2 with a wide range of immune activity, which will definitely make the anti-tumor effect of TIL cells even more powerful. We succeeded in using retroviral vectors. The IL-2 gene was introduced into oligomeric TIL cells for sustained and stable expression. PCR amplification of IL-2 gene confirmed that exogenous IL-2 gene was successfully inserted into TIL cells with a transfection rate of 1 ～