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目的观察微囊化牛嗜铬细胞（ＢＣＣ）大鼠脑内移植的效果及微囊的作用。方法将微囊化或非微囊化ＢＣＣ和空微囊分别移植于帕金森病（ＰＤ）样大鼠脑纹状体内，观察术后阿朴吗啡诱发大鼠异常旋转行为的变化；用蔗糖磷钾酸乙醛酸（ＳＰＧ）荧光染色和ＨＥ染色观察脑组织中植入的ＢＣＣ及微囊的状态。结果空囊组ＰＤ样大鼠异常旋转行为改变不明显（ｎ＝６，Ｐ＞０．０５）；非微囊组１６只ＰＤ样大鼠中９只大鼠移植后１周旋转圈数下降到移植前的４４．０％～６０．９％（Ｐ＜０．０１），移植６个月时仍有ＢＣＣ在部分受体脑内存活，移植区有较明显的炎性反应，另有７只ＰＤ样大鼠移植后异常旋转行为无明显改善（Ｐ＞０．０５），其脑内未见存活的ＢＣＣ，移植区有较明显的炎性反应；微囊组１６只ＰＤ样大鼠移植后旋转圈数下降到移植前的１７．６％～３５．６％（Ｐ＜０．０１），移植后１０个月时大鼠脑内仍存在大量存活的微囊化ＢＣＣ，无明显的炎性反应。微囊化ＢＣＣ移植改善ＰＤ样大鼠异常旋转行为的效果明显优于非微囊者（Ｐ＜０．０１）。结论ＢＣＣ脑内移植可改善ＰＤ样大鼠的异常旋转行为；大鼠旋转行为的改善与ＢＣＣ在脑内的存活状态有关；海藻酸钠及多聚赖氨酸制作的微囊可降低异种ＢＣＣ?
Objective To observe the effects of intracerebral transplantation of microencapsulated bovine chromaffin cells (BCC) and the effects of microcapsules. Methods Microencapsulated or non-microencapsulated BCC and empty microcapsules were transplanted into the striatum of Parkinson’s disease (PD) -like rats, and the changes of abnormal rotation behavior of apomorphine-induced rats were observed. Phosphoric acid glyoxylate (SPG) fluorescence staining and HE staining observed in brain tissue implanted BCC and microcapsule status. Results There was no significant change in the abnormal rotation behavior of PD-like rats in the balloon group (n = 6, P> 0.05). In the non-microcapsule group, the number of rotations of one of the 16 PD rats decreased to 44.0% -60.9% (P <0.01) before transplantation. BCC still survived in some recipients at 6 months after transplantation, and there were more obvious inflammatory reactions in the graft area. Another 7 PD There was no significant improvement in abnormal rotation behavior after transplantation in rats (P> 0.05). No BCC survived in the brain and more inflammatory reaction in the transplantation area. After transplantation, 16 PD rats in the microcapsule group were rotated The number of laps decreased from 17.6% to 35.6% before transplantation (P <0.01). At 10 months after transplantation, there was still a large number of surviving microencapsulated BCC in the rat brain, with no obvious inflammatory reaction . The effect of microencapsulated BCC transplantation on improving the abnormal rotation behavior of PD-like rats was significantly better than that of non-microencapsulated ones (P <0.01). Conclusions The intracerebral transplantation of BCC can improve the abnormal rotation behavior of PD-like rats. The improvement of the rotational behavior of rats is related to the survival status of BCC in the brain. The microcapsules prepared by sodium alginate and polylysine can reduce the heterogeneous BCC.