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目的:探讨依那普利对大鼠单侧输尿管梗阻再通模型肾脏纤维化的影响。方法:18只SD大鼠随机分为两组:假手术组(6只)以及单侧输尿管梗阻模型组(12只)。输尿管梗阻3天后,实施梗阻再通手术,再将大鼠随机分为模型组(6只)以及依那普利组(6只),术后,依那普利组给予依那普利灌胃10mg/kg/d,假手术组以及模型组给予等量0.5%CM-CNa溶液灌胃。用药2周后,取术侧肾组织做HE染色,并采用Raford评分系统对肾间质损伤程度进行评分;用Real-timePCR方法检测I、III型胶原以及CT-GFmRNA的表达;用Westernblot方法检测CTGF蛋白水平的表达。结果:模型组大鼠肾脏损伤程度,I、III型胶原mRNA表达水平,以及CTGFmRNA和蛋白表达水平均比假手术组明显上升(P<0.01)。经依那普利治疗后,与模型组相比,以上指标均显著下降(P<0.01)。结论:依那普利能有效阻止大鼠单侧输尿管梗阻再通后肾脏纤维化的进展。依那普利抗纤维化的作用机制可能与抑制CTGF的表达有关。
Objective: To investigate the effect of enalapril on renal fibrosis in rats with unilateral ureteral obstruction. Methods: Eighteen SD rats were randomly divided into two groups: sham operation group (n = 6) and unilateral ureteral obstruction model group (n = 12). Three days after ureteral obstruction, an obstruction reoperation was performed. The rats were then randomly divided into model group (n = 6) and enalapril group (n = 6). Postoperative enalapril group was given enalapril 10mg / kg / d, sham operation group and model group given the same amount of 0.5% CM-CNa solution gavage. After 2 weeks of treatment, the kidneys were harvested for HE staining and Raford scoring system was used to evaluate the severity of renal interstitial injury. Real-time PCR was used to detect the expression of type I and type III collagen and CT-GF mRNA. Western blot was used to detect CTGF protein expression. Results: The renal injury, the expression of type I and type III collagen mRNA and the expression of CTGF mRNA and protein in model group were significantly higher than those in sham operation group (P <0.01). After enalapril treatment, compared with the model group, the above indicators were significantly decreased (P <0.01). Conclusion: Enalapril can effectively prevent the progression of renal fibrosis after unilateral ureteral obstruction in rats. The mechanism of anti-fibrosis of enalapril may be related to the inhibition of CTGF expression.