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通过在三种杨树无性系,I-214(Populus×euranericanacv.I-214)(ltalica)、中东杨(P.berolinensis)(Berolinensis)和群众杨(P.popularis35-44)(Popularis)一年生盆栽插条苗的木质部导入ABA和细胞分裂素,研究了这两类激素在气孔调控中的作用。尽管不同无性系的气孔在对ABA的敏感性上存在显著差异,但ABA仍可导致气孔的关闭,然而在蒸腾流中的细胞分裂素(与ABA共导入或分别导入)可以明显地抑制ABA的作用。并且玉米素还能推迟土壤干旱所诱导的气孔关闭,在水分胁迫条件下,内源细胞分裂素浓度下降而同时ABA上升.据此提出了复合胁迫信号的概念,即在根冠通讯中,是ABA和细胞分裂素共同调控气孔的运动。另外还研究了玉米素、激动素、6-BA等不同细胞分裂素与ABA的相互作用,结果发现6-BA与玉米素和激动素的作用相反,它不能抑制ABA的作用,反而促进其对气孔的关闭
An annual pot experiment was conducted in three poplar clones, I-214 (ltalica, P.berolinensis and P. popularis) The xylem of cuttings was introduced into ABA and cytokinin, and the roles of these two hormones in stomatal regulation were studied. ABA can still cause stomatal closure despite significant differences in stomatal sensitivity to ABA, whereas cytokinin in transpiration (co-introduced with ABA or introduced separately) significantly inhibits ABA effect. Zeatin also delayed the stomatal closure induced by soil drought. Under water stress conditions, the concentration of endogenous cytokinin decreased while ABA increased. Accordingly, the concept of composite stress signal is proposed, that is, in root-crown communication, it is the movement of stomatal joint controlled by ABA and cytokinin. In addition, the interaction between different cytokinin such as zeatin, kinetin, 6-BA and ABA was also studied. It was found that 6-BA, on the contrary to zeatin and kinetin, could not inhibit the action of ABA, Stomatal closure